Iindaba - Imveliso yeNdawo yeNdalo evela kwizibonelelo ezihlaziyiweyo

I.intuve

I-Vanillin yenye yezona zinto ziphakanyisiweyo kwaye zisetyenziswa ngokubanzi emhlabeni. Ngokwesiko, ikhutshiwe kwiimbotyi ze-vanilla, ezixabisa kakhulu kwaye zijongana nemiceli mngeni malunga nokuzinza kunye nokubonelela ngempandla. Nangona kunjalo, ngenkqubela phambili kwi-biotechnology, ngakumbi kwicandelo le-biotranform ye-birbial, ixesha elitsha lemveliso ye-evallin yendalo ivele. Sebenzisa i-microorganisms yenguqu yendalo yezinto eziluhlaza zendalo ibonelele ngendlela efanelekileyo yendlela ye-synthesis ye-vanillin. Le ndlela ayiphendulanga kuphela iinkxalabo ezizinzileyo kodwa ikwabonelela ngezisombululo ezinobuchule kwishishini lencasa. Uphando olwenziwe liziko le-SRM kunye neTekhnoloji (i-srmast) ibonelele ngovavanyo olubanzi lweendlela ze-vantntin kunye nezicelo zazo kwicandelo leendawo zokutya, kwizicelo zawo ezahlukeneyo kwishishini lokutya.

II. Ungayifumana njani iNdawo yeNdalo evela kwizibonelelo ezihlaziyiweyo

UKUSETYENZISWA KOKUSETYENZISELWA I-FID

I-Ferulic Acid, ethathwe kwimithombo yolwazi njengerayisi kunye ne-oat ye-oat, ibonakalisa izinto ezifanayo ze-vallin kwaye isebenza njengemveliso esetyenziswayo ende e-varillin. Ii-microorganisms ezahlukeneyo ezinjenge-pseudmonas, i-Aspergills, ii-streptomys, kunye nefungi zisetyenziselwe ukuveliswa kwe-vallin kwiferilic acid. Ngokukodwa, iintlobo ezifana neAmycophatopsis kunye ne-fungi ye-White-Botf ichongiwe njengabagqatswa abanokubakho ngokuvelisa i-valilic acid. Izifundo ezininzi ziye zaphanda ngemveliso ye-vanlin evela kwi-ferulic acid isebenzisa i-microorganism, iindlela ezinokuthengiswa, kunye neenkqubo ezingenayo, zibonisa ukungahambi kakuhle kunye nokubakho kwale ndlela.

I-enzymatic synthesis ye-vantlin evela kwi-ferulic acid ibandakanya i-Enzyme feruloyl esterase, ekhulisa i-hydrolysis ye-fldrolysis, ikhupha i-vanllin kunye nezinye iimveliso ezinxulumene noko. Ngokuvavanya ubungakanani obulungileyo bee-enzynts ze-billin bioynthicic kwiinkqubo ezingenalfowuni, abaphandi baphuhlise i-Escherictinia ephuculweyo ye-coli. Ukongeza, ukusetyenziswa kwe-microfility ye-microbial ye-microbial ithathele ingqalelo ngenxa ye-bi biomatining yayo kunye nokuzinza phantsi kweemeko ezahlukeneyo. Indlela yokungalawuleki yethamo ye-vanlilin kwimveliso ye-valelin kwi-ferulic acid iphuhlisiwe, isuse imfuno ye-coenazmes. Le ndlela ibandakanya i-coenzyme iredylase ye-REEDYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYYNSY NE-COENYYME-I-OXYGENDA UKUGCINWA KWE-OXYGENDA UKUGCINWA KOKUGCINWA KWE-FERLuD Acid kwi-vanlin. I-CO-ARMOORDONOMOORD ye-FDC kunye ne-CSSO2 zenza ukuba ukuveliswa kwe-2,5 mg ye-vallin evela kwi-ferulic acid kwi-cuscles fuds, iphawula imeko ye-enzyme ye-enzyme engalawuleki.

Ukusetyenziswa kwe-Eugenol / Isoeugenol njengendawo

I-Eugenol kunye ne-isoeugenol, xa ziphantsi kwe-bhactoconversion, zavelisa i-vanlin kunye neMetabolites zayo ezinxulumene neMetaboli, ezifunyenweyo zokongezwa kwezicelo kunye nexabiso lezoqoqosho elibalulekileyo. Izifundo ezininzi ziye zavavanya ukusetyenziswa kwemfuza yemfuza yemvelo kunye nendalo yenzeka ngokwendalo ukuze isebenze i-eugenol. Amandla e-Eugenol aqwalaselwe kwiibhaktiriya ezahlukeneyo kunye nefungi, kubandakanya kodwa engaphelelanga kwi-bacils, i-pseudmonas, i-Assergilsus, neRhodomonas, kunye ne-Rhodomonis, ebonisa ukuveliswa kwabo kwimveliso ye-Eugenol-vanlin evaliweyo. Ukusetyenziswa kwe-Eugenol Oxidase (i-EUGO) njenge-enzyme yemveliso eVallin kwinkqubo yemveliso ibonakalise amandla afanelekileyo. U-Eugo ubonakalisa uzinzo kunye nomsebenzi ngaphezulu kwe-PH ye-PH, ngokunyibilika umsebenzi owonyusayo kunye nokunciphisa ixesha lokuphendula. Ngapha koko, ukusetyenziswa kwe-EUGO engafakwanga i-EUGO ivumela ukubuyiselwa kwe-biocutalyst ukuya kuthi ga kwi-18 yemijikelezo yokuphendula, ekhokelela ekunyukeni okungaphezulu kwe-12. Ngokufanayo, i-Enzyme Cso2 engafakwanga i-CSO2 inokukhuthaza ukuguqulwa kwe-isoeugenol kwi-vanlin ngaphandle kokuthembela kwi-coenazmes.

Abanye abantu

Ukongeza kwi-ferulic acid kunye ne-Eugenol, ezinye iziqu ezifana ne-vanillic acid kunye ne-C6-C3 Phenylpropanoids zichongiwe njengemveliso ye-vallin. I-Vanillic Acid, iveliswa njengemveliso ye-LIGINS okanye njengecandelo elikhuphisana neMetabol Indlela, ithathwa njengeyona nto iphambili kwimveliso ye-biilin esekwe kwi-bio-ivaler. Ngaphaya koko, ukubonelela ngokuqonda ekusetyenzisweni kwe-C6-C3 phenylpropanoids ye-vanlin synthesis inika ithuba elikhethekileyo lokuphumelela okuzinzileyo kunye nobuchule.

Ukuqukumbela, ukusetyenziswa kwezixhobo ezinokuhlaziywa kwemveliso ye-vanlin yendalo ngokuphumelela kwe-nimbial yindawo yomhlaba ophucukileyo kwishishini lencasa. Le ndlela ibonelela ngenye indlela, indlela ezizinzileyo yokuveliswa kweVanillin, ilungisa iinkxalabo ezizinzileyo kunye nokunciphisa ukuthembela ngeendlela zokukhupha. Izicelo ezahlukeneyo kunye nexabiso loqoqosho le-vanlin kulo lonke ishishini lokutya ligxininisa ukubaluleka kokuqhubeka kophando kunye nophuhliso kule ndawo. Inkqubela phambili yexesha elizayo kwicandelo le-van vanlin yendalo inakho ukuguqula ishishini lencasa, ukubonelela ngeendlela ezizinzileyo nezinezinto ezizinzileyo nezendalo. Njengoko siqhubeka nokusebenzisa amandla esibonelelo esivuselelekileyo kunye nokuveliswa kwe-bietechnologion, ukuveliswa kwendalo ePhalilin ukusuka kumanzi ahlukeneyo abonisa i-avenue ethembisayo ngenxa yemveliso enokuyifumana.

III. Zithini izibonelelo zokusebenzisa izixhobo ezihlaziyiweyo zokuvelisa i-vanlin yendalo

Indawo intle:Sebenzisa izixhobo ezihlaziyiweyo ezinjengezityalo kunye nenkunkuma ye-biomass ukuvelisa i-vanlin fulls, ukunciphisa iimpembelelo ezimbi kwindalo, kunye nokunciphisa ukukhutshwa kwegesi yeGreenhouse.

Ukuzinza:Ukusebenzisa izixhobo ezihlaziyiweyo kwenza ukuba izinto ezizinzileyo zezinto ezizinzileyo kunye nezinto ezingafunekiyo, ukunceda ukukhusela ubutyebi bendalo kwaye zifezekise iimfuno zesizukulwana esizayo.

Ukukhuselwa kwezinto ezahlukeneyo zenzululwazi:Ngokusetyenziswa kwezixhobo ezihlaziyiweyo, izixhobo zokutyala zasendle zinokukhuselwa, ezinegalelo kukhuseleko lwezinto ezahlukeneyo zenzululwazi kunye nokugcinwa kwendalo.

Umgangatho wemveliso:Xa kuthelekiswa ne-synthetic vanlin, i-vanlin yendalo inokuba nenzuzo engakumbi kwi-aroma esemgangathweni kunye neempawu zendalo, eziza kunceda ukuphucula umgangatho weemveliso zencasa kunye neemveliso zenkozo.

Ukucutha ukuxhomekeka kwi-fossil fuels:Ukusetyenziswa kwezixhobo ezihlaziyiweyo kuyanceda ukunciphisa ukuxhomekeka kwi-fuels ye-fossil ye-Fossil, nto leyo eyingenelo yokhuseleko lwamandla kunye nokwahluka kwamandla. Ndiyathemba ukuba ulwazi olungentla luphendule imibuzo yakho. Ukuba ufuna uxwebhu lwereferensi ngesiNgesi, nceda undazise ukuze ndikunike wena.

Iv. Ukuqukumbela

Amandla okusebenzisa izixhobo ezihlaziyiweyo zokuvelisa i-vanlin yendalo njengendlela enolondolozo nelingqongileyo ibalulekile. Le ndlela ibambelela ekusombululeni isidingo esandayo se-valillin yendalo ngelixa kuncitshiswa ukuthembeka ngeendlela zokwenziwa.

I-Valilin ibambe isikhundla esibalulekileyo kwishishini lencasa, elixabiswe kwi-armaives yayo kunye nokusetyenziswa ngokubanzi njengearhente yencasa kwiimveliso ezahlukeneyo. Kubalulekile ukuba ugxininise ukubaluleka kweNdalo e-Valilin njengesithako esifuna ukutya, isiselo, kunye namashishini okuvuza ngenxa yeprojekhthi yabo ephezulu kakhulu.

Ngapha koko, intsimi yemveliso yendalo eValilin inika amathuba okufikelela kuphando nophuhliso. Oku kubandakanya ukuphonononga itekhnoloji entsha kunye neendlela ezinobuchule zokuphucula ukusebenza kunye nokuzinza okuvelisa i-vanlin yendalo kwizibonelelo ezihlaziyiweyo. Ukongeza, ukuphuculwa kweendlela zokuvelisa iindleko kwaye iindleko ziya kudlala indima ebaluleke kakhulu ekukhuliseni ukwamkelwa ngokubanzi kwendalo ye-valillin yendalo njengendawo ezinzileyo nezendalo kwishishini lencasa.